4.1

Biological molecules

Cambridge IGCSE Biology (0610)  · Unit 4: Biological molecules  · 12 flashcards

Biological molecules is topic 4.1 in the Cambridge IGCSE Biology (0610) syllabus , positioned in Unit 4 — Biological molecules .  In one line: Carbohydrates are composed of carbon, hydrogen, and oxygen. Examples include glucose (C6H12O6) and starch, which are vital energy sources for organisms like plants and animals.

This topic is examined in Paper 1 (multiple-choice) and Papers 3/4 (theory), plus Paper 5 or Paper 6 (practical / alternative to practical). Past papers from 2022 to 2025 show this topic across 14 questions worth 183 marks (around 2.7% of all Biology marks in those years).

The deck below contains 12 flashcards — 4 definitions, 3 key concepts and 5 process cards — covering the precise wording mark schemes reward.  Use the 4 definition cards to lock down command-word answers (define, state), then move on to the concept and application cards to handle explain, describe and compare questions.

Key definition

What chemical elements make up carbohydrates

Carbohydrates are composed of carbon, hydrogen, and oxygen. Examples include glucose (C6H12O6) and starch, which are vital energy sources for organisms like plants and animals.

What the Cambridge 0610 syllabus says

Official 2026-2028 spec

These are the exact learning objectives Cambridge sets for this topic. Match the command word (Describe, Explain, State, etc.) in your answer to score full marks.

  1. List List the chemical elements that make up: carbohydrates, fats and proteins
  2. State State that large molecules are made from smaller molecules, limited to: (a) starch, glycogen and cellulose from glucose (b) proteins from amino acids (c) fats and oils from fatty acids and glycerol
  3. Describe Describe the use of: (a) iodine solution test for starch (b) Benedict's solution test for reducing sugars (c) biuret test for proteins (d) ethanol emulsion test for fats and oils (e) DCPIP test for vitamin C
  4. Describe Describe the structure of a DNA molecule: (a) two strands coiled together to form a double helix (b) each strand contains chemicals called bases (c) bonds between pairs of bases hold the strands together (d) the bases always pair up in the same way: A with T, and C with G (full names are not required) Supplement
Definition Flip

What chemical elements make up carbohydrates?

Answer Flip

Carbohydrates are composed of carbon, hydrogen, and oxygen. Examples include glucose (C6H12O6) and starch, which are vital energy sources for organisms like plants and animals.

Definition Flip

What chemical elements make up fats?

Answer Flip

Fats are composed of carbon, hydrogen, and oxygen, but with a lower proportion of oxygen compared to carbohydrates. An example is triglycerides, which are important for energy storage in adipose tissue in mammals.

Definition Flip

What chemical elements make up proteins?

Answer Flip

Proteins are composed of carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.

Example: enzymes are proteins that catalyze biochemical reactions in organisms like the digestion of food in the human stomach.
Key Concept Flip

What smaller molecules make up starch, glycogen, and cellulose?

Answer Flip

Starch, glycogen, and cellulose are all made from glucose molecules. Starch is found in plants (

Example: potatoes), glycogen in animal liver and muscle cells, and cellulose forms plant cell walls, providing structure to plants.
Key Concept Flip

What smaller molecules make up proteins?

Answer Flip

Proteins are made from amino acids. These amino acids link together via peptide bonds to form polypeptide chains. For instance, haemoglobin in red blood cells is a protein made from amino acids.

Key Concept Flip

What smaller molecules make up fats and oils?

Answer Flip

Fats and oils are made from fatty acids and glycerol. These combine to form triglycerides.

Example: fats stored in seeds provide energy for germination, and oils like olive oil are used for cooking.
Key Concept Flip

Describe the iodine solution test and its expected result for starch.

Answer Flip

Add iodine solution to the sample. If starch is present, the iodine solution will change from brown to blue-black.

Example: testing a potato slice will result in a blue-black color due to the presence of starch.
Key Concept Flip

Describe the Benedict's solution test and its expected result for reducing sugars.

Answer Flip

Add Benedict's solution to the sample and heat. If reducing sugars are present, the solution will change color from blue to green, yellow, orange, or brick red, depending on the concentration of sugar.

Example: testing glucose solution will result in a brick-red color if the glucose concentration is high.
Key Concept Flip

Describe the Biuret test and its expected result for proteins.

Answer Flip

Add Biuret reagent to the sample. If protein is present, the solution will change from blue to purple.

Example: testing egg white will result in a purple color because of its high protein content.
Key Concept Flip

Describe the ethanol emulsion test and its expected result for fats and oils.

Answer Flip

Dissolve the sample in ethanol, then add water. If fats or oils are present, a cloudy white emulsion will form.

Example: testing milk will result in a cloudy white emulsion due to the presence of fat.
Key Concept Flip

Describe the DCPIP test and its expected result for vitamin C.

Answer Flip

Add the sample dropwise to DCPIP solution until the blue colour disappears. The volume of sample needed indicates the amount of vitamin C. Lemon juice will quickly decolourise DCPIP due to high vitamin C content.

Definition Flip

Describe the structure of a DNA molecule.

Answer Flip

DNA consists of two strands coiled together to form a double helix. Each strand contains bases (A, T, C, G). Bases pair up specifically: A with T, and C with G, held together by bonds between the base pairs. The sequence of these bases encodes genetic information in organisms, like eye colour in humans.

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Key Questions: Biological molecules

What chemical elements make up carbohydrates?

Carbohydrates are composed of carbon, hydrogen, and oxygen. Examples include glucose (C6H12O6) and starch, which are vital energy sources for organisms like plants and animals.

What chemical elements make up fats?

Fats are composed of carbon, hydrogen, and oxygen, but with a lower proportion of oxygen compared to carbohydrates. An example is triglycerides, which are important for energy storage in adipose tissue in mammals.

What chemical elements make up proteins?

Proteins are composed of carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.

Example: enzymes are proteins that catalyze biochemical reactions in organisms like the digestion of food in the human stomach.
Describe the structure of a DNA molecule.

DNA consists of two strands coiled together to form a double helix. Each strand contains bases (A, T, C, G). Bases pair up specifically: A with T, and C with G, held together by bonds between the base pairs. The sequence of these bases encodes genetic information in organisms, like eye colour in humans.

Tips to avoid common mistakes in Biological molecules

Cambridge syllabus keywords to use in your answers

These are the official Cambridge 0610 terms tagged to this section. Mark schemes credit responses that use the exact term — weave them into your answers verbatim rather than paraphrasing.

carbohydrate protein lipid fat oil starch glucose glycogen cellulose amino acid fatty acid glycerol polymer monomer maltose sucrose Benedict's test iodine test biuret test emulsion test reducing sugar non-reducing sugar

Key terms covered in this Biological molecules deck

Every term below is defined in the flashcards above. Use the list as a quick recall test before your exam — if you can't define one of these in your own words, flip back to that card.

What chemical elements make up carbohydrates
What chemical elements make up fats
What chemical elements make up proteins
Describe the structure of a DNA molecule

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