12.3

Identification of ions and gases

Cambridge IGCSE Chemistry (0620)  · Unit 12: Experimental techniques and chemical analysis  · 11 flashcards

Identification of ions and gases is topic 12.3 in the Cambridge IGCSE Chemistry (0620) syllabus , positioned in Unit 12 — Experimental techniques and chemical analysis , alongside Experimental design and Separation and purification.  In one line: Paper chromatography is used to separate mixtures of soluble colored or colorless substances using a suitable solvent. It relies on the different solubilities and affinities of the substances for the stationary (paper) and mobile (solvent) phases.

This topic is examined in Paper 1 (multiple-choice) and Papers 3/4 (theory), plus Paper 5 or Paper 6 (practical / alternative to practical). Past papers from 2022 to 2025 show this topic across undefined questions worth 83 marks (around 1.3% of all Chemistry marks in those years).

The deck below contains 11 flashcards — 3 definitions, 1 key concept and 2 identification cards — covering the precise wording mark schemes reward.  Use the 3 definition cards to lock down command-word answers (define, state), then move on to the concept and application cards to handle explain, describe and compare questions.

Key definition

Paper chromatography used for

Paper chromatography is used to separate mixtures of soluble colored or colorless substances using a suitable solvent. It relies on the different solubilities and affinities of the substances for the stationary (paper) and mobile (solvent) phases.

What the Cambridge 0620 syllabus says

Official 2026-2028 spec

These are the exact learning objectives Cambridge sets for this topic. Match the command word (Describe, Explain, State, etc.) in your answer to score full marks.

  1. Describe Describe how paper chromatography is used to separate mixtures of soluble coloured substances, using a suitable solvent
  2. Interpret Interpret simple chromatograms to identify: (a) unknown substances by comparison with known substances (b) pure and impure substances
  3. Describe Describe how paper chromatography is used to separate mixtures of soluble colourless substances, using a suitable solvent and a locating agent Supplement
  4. State State and use the equation for R: distance travelled by substance R= distance travelled by solvent Supplement
Definition Flip

What is paper chromatography used for?

Answer Flip

Paper chromatography is used to separate mixtures of soluble colored or colorless substances using a suitable solvent. It relies on the different solubilities and affinities of the substances for the stationary (paper) and mobile (solvent) phases.

Key Concept Flip

How can paper chromatography identify unknown substances?

Answer Flip

Unknown substances can be identified by comparing their chromatograms to those of known substances run under identical conditions. Substances with matching Rf values are likely the same.

Key Concept Flip

How can paper chromatography indicate the purity of a substance?

Answer Flip

A pure substance will produce a single spot on the chromatogram. An impure substance will produce multiple spots, indicating the presence of different components.

Definition Flip

What is a locating agent, and when is it used?

Answer Flip

A locating agent is a substance used to make colorless substances visible on a chromatogram. It reacts with the colorless substances to produce colored spots. Ninhydrin is a common locating agent for amino acids.

Key Concept Flip

State the equation for calculating the Rf value.

Answer Flip

Rf = (distance travelled by substance) / (distance travelled by solvent). The Rf value is a characteristic constant for a given substance under specific chromatographic conditions.

Key Concept Flip

A spot travels 3.0 cm on a chromatogram, and the solvent front travels 5.0 cm. Calculate the Rf value.

Answer Flip

Rf = (distance travelled by substance) / (distance travelled by solvent) = 3.0 cm / 5.0 cm = 0.6. Remember to include units for working, but the Rf value itself is dimensionless.

Key Concept Flip

Why is it important to use the same conditions when comparing chromatograms?

Answer Flip

Rf values are affected by factors such as the solvent used, the temperature, and the type of paper. To accurately compare, all variables except the unknown substance must be constant.

Definition Flip

What do the state symbols (s), (aq), (l), and (g) represent?

Answer Flip

(s) represents solid, (aq) represents aqueous (dissolved in water), (l) represents liquid, and (g) represents gas. All state symbols must be correct in chemical equations.

Key Concept Flip

In a reaction, 25.0 cm³ of 0.400 mol/dm³ HCl reacts completely. How many moles of HCl are used?

Answer Flip

Moles of HCl = (Volume in dm³/1000) x Concentration = (25.0/1000) x 0.400 = 0.0100 mol.

Key Concept Flip

If 0.0100 mol of HCl reacts according to the equation 2HCl + CaCO₃ → CaCl₂ + H₂O + CO₂, how many moles of CO₂ are produced?

Answer Flip

From the balanced equation, 2 moles of HCl produce 1 mole of CO₂. So, moles of CO₂ = 0.0100 mol HCl / 2 = 0.00500 mol.

Key Concept Flip

If 0.00500 mol of CO₂ gas is produced, what volume does it occupy at room temperature and pressure (RTP), assuming 1 mole of gas occupies 24000 cm³?

Answer Flip

Volume of CO₂ = Moles of CO₂ x Molar volume at RTP = 0.00500 mol x 24000 cm³/mol = 120 cm³.

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12.2 Separation and purification

Key Questions: Identification of ions and gases

What is paper chromatography used for?

Paper chromatography is used to separate mixtures of soluble colored or colorless substances using a suitable solvent. It relies on the different solubilities and affinities of the substances for the stationary (paper) and mobile (solvent) phases.

What is a locating agent, and when is it used?

A locating agent is a substance used to make colorless substances visible on a chromatogram. It reacts with the colorless substances to produce colored spots. Ninhydrin is a common locating agent for amino acids.

What do the state symbols (s), (aq), (l), and (g) represent?

(s) represents solid, (aq) represents aqueous (dissolved in water), (l) represents liquid, and (g) represents gas. All state symbols must be correct in chemical equations.

Tips to avoid common mistakes in Identification of ions and gases

More topics in Unit 12 — Experimental techniques and chemical analysis

Identification of ions and gases sits alongside these Chemistry decks in the same syllabus unit. Each uses the same spaced-repetition system, so progress in one informs the next.

Cambridge syllabus keywords to use in your answers

These are the official Cambridge 0620 terms tagged to this section. Mark schemes credit responses that use the exact term — weave them into your answers verbatim rather than paraphrasing.

test flame test sodium hydroxide test precipitate cation anion carbonate test sulfate test halide test ammonium test gas test hydrogen test oxygen test carbon dioxide test chlorine test ammonia test limewater splint squeaky pop relights bleaches

Key terms covered in this Identification of ions and gases deck

Every term below is defined in the flashcards above. Use the list as a quick recall test before your exam — if you can't define one of these in your own words, flip back to that card.

Paper chromatography used for
Locating agent, and when is it used
What do the state symbols (s), (aq), (l), and (g) represent

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