12.1 A2 Level

Energy

Cambridge A-Level Biology (9700)  · Unit 12: Energy and respiration  · 8 flashcards

Energy is topic 12.1 in the Cambridge A-Level Biology (9700) syllabus , positioned in Unit 12 — Energy and respiration , alongside Respiration.  In one line: 1. Cytoplasm: Substrate-linked reactions. 2. Mitochondria/Chloroplasts: Chemiosmosis in membranes.

Marked as A2 Level: examined at A Level in Paper 4 (A Level Structured Questions) and Paper 5 (Planning, Analysis and Evaluation). It is not tested on the AS-only papers (Papers 1, 2 and 3).

The deck below contains 8 flashcards — 2 definitions, 5 key concepts and 1 calculation — covering the precise wording mark schemes reward.  Use the 2 definition cards to lock down command-word answers (define, state), then move on to the concept and calculation cards to handle explain, describe, calculate and compare questions.

Key definition

Two cellular locations where ATP is synthesized

1. Cytoplasm: Substrate-linked reactions. 2. Mitochondria/Chloroplasts: Chemiosmosis in membranes.

What the Cambridge 9700 syllabus says

Official 2025-2027 spec · A2 Level

These are the exact learning outcomes Cambridge sets for this topic. The candidate is expected to be able to do each of these on the relevant paper.

  1. outline the need for energy in living organisms, as illustrated by active transport, movement and anabolic reactions, such as those occurring in DNA replication and protein synthesis
  2. describe the features of ATP that make it suitable as the universal energy currency
  3. state that ATP is synthesised by: • transfer of phosphate in substrate-linked reactions • chemiosmosis in membranes of mitochondria and chloroplasts
  4. explain the relative energy values of carbohydrates, lipids and proteins as respiratory substrates
  5. state that the respiratory quotient (RQ) is the ratio of the number of molecules of carbon dioxide produced to the number of molecules of oxygen taken in, as a result of respiration
  6. calculate RQ values of different respiratory substrates from equations for respiration
  7. describe and carry out investigations, using simple respirometers, to determine the RQ of germinating seeds or small invertebrates (e.g. blowfly larvae)

Cambridge syllabus keywords to use in your answers

These are the official Cambridge 9700 terms tagged to this section. Mark schemes credit responses that use the exact term — weave them into your answers verbatim rather than paraphrasing.

anabolic reactions ATP substrate-linked reactions chemiosmosis respiratory substrates respiratory quotient

Tips to avoid common mistakes in Energy

Key Concept Flip

Outline three energy-requiring processes in living organisms.

Answer Flip

1. Active Transport: Moving substances against their concentration gradient. 2. Movement: Muscle contraction requires ATP. 3. Anabolic Reactions: Synthesis of complex molecules like DNA replication and protein synthesis.

Key Concept Flip

Describe three features of ATP that make it suitable as the universal energy currency.

Answer Flip

1. Small, manageable energy packets: Releases energy in small, usable amounts. 2. Readily Hydrolyzed: Phosphate bonds are easily broken to release energy. 3. Renewable: Quickly regenerated from ADP and Pi using energy from respiration or photosynthesis.

Definition Flip

State two cellular locations where ATP is synthesized.

Answer Flip

1. Cytoplasm: Substrate-linked reactions. 2. Mitochondria/Chloroplasts: Chemiosmosis in membranes.

Key Concept Flip

Explain the relative energy values of carbohydrates, lipids, and proteins as respiratory substrates.

Answer Flip

Lipids have the highest energy value due to their higher proportion of C-H bonds, which release more energy upon oxidation. Proteins have a variable energy value, while carbohydrates have the lowest energy value per unit mass.

Definition Flip

Define the Respiratory Quotient (RQ).

Answer Flip

The Respiratory Quotient (RQ) is the ratio of the number of molecules of carbon dioxide produced to the number of molecules of oxygen consumed during respiration. RQ = CO₂ produced / O₂ consumed.

Calculation Flip

Calculate the RQ value for the complete oxidation of glucose (C₆H₁₂O₆).

Answer Flip

The equation is: C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O. RQ = CO₂ produced / O₂ consumed = 6/6 = 1.0

Key Concept Flip

Describe the purpose of using KOH or soda lime in a respirometer experiment.

Answer Flip

KOH or soda lime is used to absorb the carbon dioxide produced by the respiring organism (

Example: germinating seeds or invertebrates). This allows for accurate measurement of oxygen consumption as the change in volume is solely due to oxygen uptake.
Key Concept Flip

Explain how a respirometer measures the rate of respiration.

Answer Flip

As the organism respires and consumes oxygen, the volume of gas in the respirometer decreases. This decrease in volume causes the fluid in the manometer to move. The distance the fluid moves in a given time is a measure of the rate of oxygen consumption, and thus, the rate of respiration.

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More topics in Unit 12 — Energy and respiration

Energy sits alongside these A-Level Biology decks in the same syllabus unit. Each uses the same spaced-repetition system, so progress in one informs the next.

Key terms covered in this Energy deck

Every term below is defined in the flashcards above. Use the list as a quick recall test before your exam — if you can't define one of these in your own words, flip back to that card.

Two cellular locations where ATP is synthesized
The Respiratory Quotient (RQ)

How to study this Energy deck

Start in Study Mode, attempt each card before flipping, then rate Hard, Okay or Easy. Cards you rate Hard come back within a day; cards you rate Easy push out to weeks. Your progress is saved in your browser, so come back daily for 5–10 minute reviews until every card reads Mastered.